產(chǎn)品英文名稱：BHK HCP ELISA kit
產(chǎn)品中文名稱：BHK 細(xì)胞 ELISA 試劑盒

BHK HCP ELISA kit 的應(yīng)用
本試劑盒是用于確定在BHK細(xì)胞中表達(dá)的制成品的宿主細(xì)胞蛋白污染的存在。該試劑盒是用于研究和制造使用，不用于人類或動(dòng)物的診斷應(yīng)用。

：，客服

BHK 細(xì)胞 ELISA 試劑盒的英文描述

The BHK (Baby Hamster Kidney Fibroblast) cell line is used as an expression system for viral vaccines and other therapeutic proteins. Our HCP kit is generic in the sense that it is intended to react with essentially all of the HCPs that could contaminate the product independent of the purification process. The antibodies have been generated against and affinity purified using a mild lysate of BHK cells. The resulting antibodies have then been characterized against 3 commercial cell lines used to produce various viral and protein products. This analysis indicated the vast majority of HCPs were conserved among multiple BHK cell lines and product purification processes. Our assay validation has determined that the LOD for this kit is ~0.4ng/mL.

BHK HCP ELISA ki t的使用步驟規(guī)則
The BHK cell assay is a two-site immunoenzymetric assay. Samples containing BHK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-BHK cell antibody (goat polyclonal) in microtiter strips coated with an
affinity purified capture goat polyclonal anti-BHK cell antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of BHK cell HCPs present.
 
BHK HCP ELISA kit 的保存和穩(wěn)定性
* Store Standards at –10°C to -30°C. All other reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.
 
BHK HCP ELISA kit 的使用注意事項(xiàng)
*只用于研究或制造。
*停止反應(yīng)試劑是0.5N  H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒(méi)有被認(rèn)為是有害的。
*本試劑盒只能由合格的技術(shù)人員使用。

一切干細(xì)胞實(shí)驗(yàn)耗材優(yōu)惠盡在上海華雅思創(chuàng)生物

2015年統(tǒng)統(tǒng)*，--一切活動(dòng)盡在上海華雅思創(chuàng)！

：，客服

StemRD   MGro-500  MSC medium,chemically-defined,間充質(zhì)干細(xì)胞培養(yǎng)基 500mL/瓶

LONZA   04-418Q    X-VIVO 15 無(wú)血清培養(yǎng)基 1L/瓶

Life Technology   087-0112DK    AIM-V® CTS™ 無(wú)血清細(xì)胞培養(yǎng)基（細(xì)胞治療級(jí)） 1000mL/瓶

Pall   15950-017  Ultroser Serum Substitute 血清替代品 20mL/瓶

LONZA   12-725F   UltraCULTURE Serum-free Medium無(wú)血清培養(yǎng)基 500mL/瓶

BD 762165   PAXgene Blood RNA Tube RNA樣本收集管 100管/盒

LONZA 50150   SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶

LONZA CC-3162   EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套

Nunc 159910   175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過(guò)濾蓋 30個(gè)/箱

LONZA LT07-318  MycoAlert® Mycoplasma Detection Kit支原體檢測(cè)試劑盒 100Tests/盒

LONZA   LT07-418 MycoAlert® Mycoplasma Detection Kit支原體檢測(cè)試劑盒 50Tests/盒

Lonza   50-650U   Kinetic-QCL 192 Test Kit內(nèi)毒素檢測(cè)試劑盒   192T/盒

Corning   431080    175cm⒉細(xì)胞培養(yǎng)瓶，Angled頸，透氣蓋 50個(gè)/箱

Corning   8388  125mL儲(chǔ)液瓶 Storage Bottles,PS,45mm瓶頸，帶蓋，滅菌，獨(dú)立包裝 24個(gè)/箱

Corning   430659    2ml凍存管, 聚丙烯，外旋蓋, 可自立圓底 500個(gè)/箱

Corning   3290     75cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 100個(gè)/箱

Corning   3292     175cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 50個(gè)/箱

BD-Falcon   352360   Cell Strainer, 100µm, Yellow 100um細(xì)胞濾網(wǎng) 50個(gè)/箱

BD Falcon 352070 50mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD Falcon 352096 15mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD BioCoat 354234   Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶

Cygnus   F550   CHO HCP ELISA Kit,3G宿主蛋白檢測(cè)試劑盒 96T/盒

Cygnus   F410   E.coli HCP ELISA Kit 大腸桿菌 96T/盒

Nalgene  5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個(gè)/箱

Nalgene    5026-0909   CryoBoxes 凍存盒 9X9 陣列，管瓶1.2/2.0mL   24個(gè)/箱

Nalgene   2250-0130 50L 可高溫高壓操作的細(xì)口大瓶（帶手柄）   1個(gè)/箱

Nalgene   2319-0050    20L 可高溫高壓滅菌的細(xì)口大瓶（帶放水口）    1個(gè)/箱

Hyclone  SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶

PeproTech   100-18B-500   Recombinant Human FGF-basic (154 a.a.)因子 500ug/支

Parafilm PM-996   Parafilm® M Laboratory Film 封口膜 12卷/箱